Computational protocol: Genetic evidence for a novel competence inhibitor in the industrially important Bacillus licheniformis

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Protocol publication

[…] Analysis of the primary protein structure of ComI was performed with TMBASE (Hofmann and Stoffel ). Sequence analysis was performed with BioEdit Evolutionary history was inferred using the Neighbor-Joining algorithm (Saitou and Nei ). The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling ) and are in the units of the number of amino acid substitutions per site. The analysis was conducted using MEGA7 (Kumar et al. ). Statistical analysis was performed with GraphPad Prism 7. […]

Pipeline specifications

Software tools BioEdit, MEGA
Databases TMBase
Application Protein structure analysis
Organisms Bacillus licheniformis, Bacillus subtilis, Bacillus licheniformis DSM 13 = ATCC 14580
Chemicals Amino Acids