Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 34
Release date: Jul 19 2018
Last update date: Nov 28 2018
Access: Public
Chemicals: Amino Acids
Genes: Crtc1, EIF2AK4
Dataset link Translational control through differential ribosome pausing during amino acid limitation in mammalian cells

Experimental Protocol


Ribosome profiling was performed in HEK293T, HCT116, or HeLa cells during limitation for leucine or arginine for 3 or 6 hours to determine the effect of limiting single amino acid levels of ribosome elongation kinetics at the cognate codons. The same cell lines grown in nutrient-rich conditions were used as a control. These experiments were repeated in HEK293T cells with 250 nM Torin1, in cells stably expressing a flag-tagged wild-type or Q99L mutant RagB-GTPase or hrGFP, and in a GCN2 knockout cell line to determine the role of the mTORC1 and GCN2 pathways.

Repositories


GEO

GSE113751

ENA

SRP143452

BioProject

PRJNA453915

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