Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Aug 31 2009
Last update date: Oct 29 2018
Access: Public
Diseases: Bronchial Diseases, Neoplasms, Respiratory Distress Syndrome, Adult, Acute Lung Injury, Ventilator-Induced Lung Injury
Genes: TNF
Dataset link Expression data from Bronchial Epithelial Cells exposed to Cyclic Stretch with and without LPS or TNFa

Experimental Protocol


Human Bronchial Epithelial Cells (Beas-B2) cells grown on silicon elastic plates coated with Type I collagen (Flexercell International, McKeesport, PA) were exposed to six regiments for 4 h: 1) control (static, [control]); 2) mechanical stretch (25 PKa, 30 cycles per min, [stretch]); 3) LPS (1 mcg/ml [LPS]); 4) TNF-α (20 ng/ml; [TNF]); 5) mechanical stretch plus LPS [LPS+S], and 6) mechanical stretch plus TNF-α [TNF+S]. Total RNA (duplicate experiments) was extracted using TRIZOL reagent (as per manufactures specifications) and purified using Qiagen mRNA purification Kit (as per manufacturers specifications). mRNA was hybridized to Affymetrix Human U133plus2.0 chips. Probe based analysis, background reduction, and quantile data normalization was performed in MeV 4.0 of TM4 using Robust Multi-array Average (RMA).

Repositories


GEO

GSE16650

ArrayExpress

E-GEOD-16650

BioProject

PRJNA116237

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Contact


Claudia dos Santos
Claudia Chimisso dos Santos