Computational protocol: The Candida albicans stress response gene Stomatin-Like Protein 3 is implicated in ROS-induced apoptotic-like death of yeast phase cells

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Protocol publication

[…] CGD ( was utilized to search for all SPFH family members in C. albicans. The Aspergillus Genome Database (AspGD, was used to retrieve SPFH sequences from A. nidulans. Sequences for human, mouse, and nematode SPFH family members were retrieved from UniProt and NCBI: HsSTOM (accession: P27105), HsSTOML1 (accession: Q9UBI4), HsSTOML2 (accession: Q9UJZ1), HsSTOML3 (accession: Q8TAV4), HsPodocin (accession: Q9NP85), MmStoml3 (accession: Q6PE84), CeStomatin2 (accession: NP_001257021.1). Sequences were aligned using T-Coffee [] with default parameters and then formatted using Boxshade ( Sequence identities and similarities between Slp3p and other SPFH family members were determined using Protein BLAST with default parameters. Post-translational modification and organelle targeting predictions were made using the bioinformatics resource portal ExPASy ( Protein schematics were constructed using Illustrator for Biological Sequences, version 1.0 []. […]

Pipeline specifications

Software tools T-Coffee, BoxShade, DELTA-BLAST
Databases AspGD ExPASy
Applications Amino acid sequence alignment, Proteome data visualization
Organisms Candida albicans, Saccharomyces cerevisiae, Homo sapiens, Caenorhabditis elegans