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Publications for Addgene
TGF β induces miR 100 and miR 125b but blocks let 7a through LIN28B controlling PDAC progression
[…] NA targeting the genomic region downstream of the AUG translation start site codon was used. Oligonucleotides containing sgRNA sequences were cloned into lentiCRISPRv2 vector (a gift from Feng Zhang; Addgene #52961) following the lentiCRISPRv2 and lentiGuide oligo cloning protocol deposited by the Zhang lab on Addgene. Oligonucleotides sequences are provided in Table . For LIN28B, miR-100 and miR- […]
Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin independent endocytosis
[…] sed for electron microscopy 24 h after transfection. Briefly, AGS cells were double transfected with GFP-tagged IRSp53 and an expression vector encoding for a GFP-binding peptide conjugated to APEX2 (Addgene plasmid #67651). Cells were washed in PBS, fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer and subjected to the 3,3′-diaminobenzidine (DAB; Sigma-Aldrich) reaction in the presen […]
Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing
[…] PCR was performed using KOD-Plus-Neo (TOYOBO, Tokyo, Japan) following the manufacturer’s protocol. To generate Prps-27::Sat2 vector (pSN444, addgene ID 108331) and Prps-27::Nat vector, the codon-optimized NTC resistance genes Sat2 (GenBank: AKT95189.1) and nourseothricin acetyl transferase (Nat) (GenBank: AAC60439.1) were synthesized by Ge […]
Propranolol sensitizes prostate cancer cells to glucose metabolism inhibition and prevents cancer progression
[…] Autophagy flux was investigated using the reporter plasmid pBABE-puro-mCherry-EGFP-LC3B (Addgene) as reported previously. Briefly, PC3 cells were transfected for 16 hours with 1 μg of plasmid per well in 6-well plates. Then, the cells were trypsinized and seeded in 8-wells micro-Slides (I […]
A genome wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve
[…] The human SOX10 open-reading frame in pDONR221 (Addgene plasmid #24749) was obtained and SOX10 was cloned into pcDNA-DEST53 using LR Clonase (ThermoFisher) to generate a GFP-SOX10 expression construct. MN1 cells  were plated at a density of 100,0 […]
Deciphering the molecular determinants of cholinergic anthelmintic sensitivity in nematodes: When novel functional validation approaches highlight major differences between the model Caenorhabditis elegans and parasitic species
[…] Hco-acr-8 coding sequence was sub-cloned into the pPD96.52 (Addgene) vector containing a myosin promoter Pmyo3, using the In-FusionHD cloning kit (Clontech). Primer sequences are listed in . C. elegans lev-8 (ok1519) were injected with 30ng μl-1 of the resulti […]
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