Computational protocol: CD47-ligation induced cell death in T-acute lymphoblastic leukemia

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Protocol publication

[…] PBS-washed cells were re-suspended in cRPMI at 4 × 105 cells/mL, incubated at 37 °C for 2 h with 125 ng/mL B6H12 or CC2C6, and fixed with BD CytoFix buffer, and labelled with Alexa Fluor488-conjugated secondary antibodies. All washes were with PBS. Labelled cells were re-suspended in Prolong Gold with DAPI (Invitrogen), mounted onto coverslips and allowed to cure overnight. Images were acquired on an Olympus IX81 microscope (20 × dry or 60 × oil objectives) equipped with a CoolSnap HQ2 camera (Photometrics) and controlled by Metamorph® software (Molecular Devices). Post-acquisition processing was performed on ImageJ. […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Application Microscopic phenotype analysis
Diseases Neoplasms, Mitochondrial Diseases, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma