Computational protocol: Gipc3 mutations associated with audiogenic seizures and sensorineural hearing loss in mouse and human

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Protocol publication

[…] To sequence coding exons in the ahl5 interval, genomic sequences were obtained from and PCR primers flanking the exons were designed using Primer3 Exons were amplified and cycle sequencing was performed as described above. Sequence chromatograms were analysed using Sequencher v4.5 (Gene Code Corporation). BLSW DNA sequences were compared against the C57BL/6J reference sequence. Presence of the Gipc3343G>A mutation was evaluated by DNA sequencing. […]

Pipeline specifications

Software tools Primer3, Sequencher
Application qPCR
Organisms Mus musculus, Homo sapiens
Diseases Genetic Diseases, Inborn, Ganglion Cysts
Chemicals Potassium