Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Dec 1 2009
Last update date: Aug 23 2018
Access: Public
Dataset link Expression data from wild type, Ring1B-/-, Eed-/-, and Ring1B/Eed double deficient mouse ES cells

Experimental Protocol


RNA from wt and PcG mutant ES cells was extracted using Trizol and hybridized to an Affymetrix Mouse 430_2.0 chip. Labeling, hybridization and primary data analysis were performed by a commercial provider (Atlas Biolabs, Berlin). Before RNA extraction, the undifferentiated ES cell state was confirmed by colony morphology. All genotypes were hybridized in biological triplicates.

Repositories


GEO

GSE19076

ArrayExpress

E-GEOD-19076

BioProject

PRJNA120481

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Contact


Markus Jaritz