Computational protocol: Identification and functional analysis of the genes encoding Δ6-desaturase from Ribes nigrum†

Similar protocols

Protocol publication

[…] Nucleotide sequences of the desaturases were aligned by using the program ClustalX 2.0.10 () and manual adjustment. The phylogenetic tree was generated using the alignment output based on the Minimum evolution method (), as implemented in the Mega 4.1 (). The complete deletion option and the Jukes and Cantor metric were used, and confidence of the tree branches was checked by bootstrap generated from 1000 replicates. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Nicotiana tabacum, Saccharomyces cerevisiae, Arabidopsis thaliana
Chemicals gamma-Linolenic Acid, Linoleic Acid