Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Feb 27 2018
Last update date: Nov 30 2018
Access: Public
Dataset link Genome-wide binding reveals intrinsic specificity and regulatory function of lineage-specifying bHLH transcription factors (RNA-Seq).

Experimental Protocol


ChIP-seq performed on murine ESCs as part of bHLH transcription factor binding screen. The antibodies used were directed against bHLH factors ASCL1, ASCL2-FLAG, and MYOD-FLAG to observe TF binding. H3K27ac enrichment was assayed using an antibody recognizing this mark for comparison to TF binding, gene expression, and chromatin accessibility from the same cell lines.

Repositories


GEO

GSE97713

ENA

SRP103844

BioProject

PRJNA382709

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Contact


Jane Johnson