Computational protocol: Clinical Role of ASCT2 (SLC1A5) in KRAS-Mutated Colorectal Cancer

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Protocol publication

[…] Cell lines were seeded into 12-well plates and grew until 80–90% confluence. Confluent cultures were scratched with sterile tips, washed with PBS, and cultured in DMEM containing 5% FBS. Cells were photographed by a 50× magnification at 0, 24, and 48 h. Wound closure (%) was evaluated using the ImageJ software. [...] All values were expressed as mean ± standard deviation (SD). Statistical analyses were conducted with the JMP Pro 12 (SAS Institute, Inc., Cary, NC, USA). Student’s t-test was used for comparing means between two groups. In clinical data, the statistical significance of differences between variables of two groups was determined by student’s t-test, chi-squared test, or Fisher’s exact test. Relapse-free survival (RFS) rates were evaluated by the Kaplan–Meier survival curve and log-rank test. All analyses were two-sided, and differences with a p value of less than 0.05 were considered statistically significant in all analyses. […]

Pipeline specifications

Software tools ImageJ, JMP Pro
Applications Miscellaneous, Microscopic phenotype analysis
Diseases Neoplasms, Colorectal Neoplasms
Chemicals Amino Acids, Glutamic Acid