Dataset features


Application: Gene expression microarray analysis
Number of samples: 22
Release date: Aug 13 2005
Last update date: Mar 16 2012
Access: Public
Diseases: Colonic Neoplasms
Chemicals: Folic Acid, Nucleotides, Oxygen, Lactic Acid
Dataset link Folic Acid study in HT-29 cells

Experimental Protocol

In the present study, large-scale gene expression analysis was performed to reveal mechanistic details of the metabolic switch from aerobic glycolysis to mitochondrial OXPHOS upon folate deficiency in HT-29 human colon cancer cells and to provide new molecular insight into the folate deficient-induced inhibition of colon cancer cell growth. Therefore, as in our previous experiment, HT-29 human colon cancer cells were cultured for 3 weeks in different types and concentrations of folate; synthetic folic acid (pteroylglutamic acid, PGA ; 10 or 100 ng/ml) or natural folate (methyl-tetrahydrofolate, MTHF; 10 or 100 ng/ml) to obtain different intracellular levels of folate. Large scale oligo arrays with 10.296 human oligo probes of which about 300 oligo probes are involved in energy metabolism, were used to study folate induced gene expression changes in order to better understand the metabolic switch and the growth inhibitory effects upon folate deficiency in HT-29 human colon cancer cells.