|Application:||Gene expression microarray analysis|
|Number of samples:||1|
|Release date:||Jan 1 2013|
|Last update date:||Apr 4 2013|
|Dataset link||Inhibition of the mitochondrial TCA cycle by monofluoroacetate in Arabidopsis thaliana|
Three independent (bio-replicate) experiments were done using three independent plant samples in the treatments. For each, approximately 30 plants were used for the treated sample and about 30 plants were used as the control sample. Plants were treated with 5 mM monofluoroacetate in 0.01% Tween 20 and incubated in the dark at room temperature (25C) for 6 hours. RNA was isolated from the inhibitor treated and control plants and used for microarray experiments. For each independent (bio-replicate) experiment, two microarrays were utilized using Cy3 and Cy5 dye-labeled samples and dye swapping was incorporated- 2 microarrays for each of 3 independent experiments (6 microarrays total).
David M. Rhoads