Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 1
Release date: Jan 1 2013
Last update date: Apr 4 2013
Access: Public
Dataset link Inhibition of the mitochondrial TCA cycle by monofluoroacetate in Arabidopsis thaliana

Experimental Protocol


Three independent (bio-replicate) experiments were done using three independent plant samples in the treatments. For each, approximately 30 plants were used for the treated sample and about 30 plants were used as the control sample. Plants were treated with 5 mM monofluoroacetate in 0.01% Tween 20 and incubated in the dark at room temperature (25C) for 6 hours. RNA was isolated from the inhibitor treated and control plants and used for microarray experiments. For each independent (bio-replicate) experiment, two microarrays were utilized using Cy3 and Cy5 dye-labeled samples and dye swapping was incorporated- 2 microarrays for each of 3 independent experiments (6 microarrays total).

Repositories


GEO

GSE29268

ArrayExpress

E-GEOD-29268

BioProject

PRJNA142935

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Contact


David Rhoads
David M. Rhoads

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