Dataset features

Specifications


Dataset type: Methylation profiling by genome tiling array
Number of samples: 48
Release date: Dec 4 2018
Last update date: Jan 10 2019
Access: Public
Dataset link Circadian oscillations of cytosine modification in humans contribute to epigenetic variability, aging, and complex disease

Experimental Protocol


Venous peripheral blood samples were collected continuously every 3 hours for 72 hours (24 samples in total) from a 52 yr old caucasian male. Neutrophils were isolated from whole blood by immunomagnetic negative selection with EasySep Direct Human Neutrophil Isolation Kit (STEMCELL) according to the manufacturer's protocol. The negative selection for neutrophils was repeated three times and the cells resuspended in phosphate buffered saline. The neutrophils were stored in -80 °C before DNA extractions. The microarray experiment was performed in technical duplicates.

Repositories


GEO

GSE83944

BioProject

PRJNA327568

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