Computational protocol: LRRK2 G2019S mutation attenuates microglial motility by inhibiting focal adhesion kinase

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Protocol publication

[…] Time-lapse images were obtained every 5 s for 15–20 min on an inverted microscope (Zeiss Xiovert 200) equipped with a × 20 objective. Stroboscopic images were generated in a 5 × 200-pixel-wide (5 s × 147 m) box drawn in the direction of cell protrusions using Metamorph software (Nashville, TN, USA). Box and whisker plots for the protrusion distance (p) and retraction distance (r) of individual events (n>20) were produced using SigmaPlot software. The middle line in each box indicates the median; the top of each box indicates the 75th percentile; the bottom indicates the 25th percentile; and the whiskers indicate the extent of the 5th and 95th percentiles. […]

Pipeline specifications

Software tools MetaMorph, SigmaPlot
Application Miscellaneous
Diseases Feline Panleukopenia, Parkinson Disease, Wounds and Injuries, Cerebrovascular Trauma
Chemicals Adenosine Diphosphate