|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Apr 4 2015|
|Last update date:||Aug 13 2018|
|Diseases:||Lung Neoplasms, Neoplasms|
|Dataset link||Diverse Targets of β-catenin during the Epithelial-Mesenchymal Transition Define Cancer Stem Cells and Predict Disease Relapse|
Total cellular RNA was extracted from LM and HM20 cells after treatment with or without Wnt3a for 24hours. Human lung cancer A549 cell–derived spheres were transferred back to adhesive tissue culture plates (LM cells). To establish an EMT/metastasis cell model, LM cells were seeded onto Matrigel-coated Boyden chamber membranes. HM20 cells were serially selected for Matrigel invasion 20 times.