Computational protocol: A new species of Potoroxyuris (Nematoda: Oxyuridae) from the woylie Bettongia penicillata (Marsupialia: Potoroidae) from southwestern Australia

Similar protocols

Protocol publication

[…] Specimens were placed in a small quantity (approximately 200 μl) of fresh glycerine alcohol in glass embryo blocks. Additional glycerine was added to clear the specimens, and gently mixed, one drop at a time, ensuring that the specimens did not collapse due to rapid osmotic pressure change. The ethanol was allowed to evaporate off over the course of a day to a week, then the specimens were mounted on glass slides in the remaining glycerine, making sure that paper or glass spacers were included under the cover slip to prevent squashing.The head ends of some specimens were cut off, mounted on slides in Hoyer’s medium, and arranged to sit mouth up as en face mounts.Photographs were taken using an Olympus BX50 microscope with an Olympus DP71 camera. Measurements were made either directly using an Olympus BH microscope equipped with an ocular micrometer, or from photographs, using ImageJ software (http://imagej.nih.gov/ij/). Drawings were made using open-source Inkscape software (www.inkscape.org) over layered photographs as a guide.Three specimens from B. penicillata Case 1 were dehydrated to absolute ethanol, critical-point dried via carbon dioxide, and sputter-coated in gold for visualization at 5 kV by a Zeiss Supra field emission scanning electron microscope at the Centre for Microscopy, Characterisation and Analysis at the University of Western Australia. […]

Pipeline specifications

Software tools ImageJ, Inkscape
Applications Miscellaneous, cryo-EM, Microscopic phenotype analysis
Organisms Nitrosomonas sp., Potorous tridactylus