Computational protocol: Comparative Functional Analysis of 12 Mammalian IFN-λ4 Orthologs

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Protocol publication

[…] IFN-λ4 protein sequences were analyzed with free online tools–Clustal W for sequence alignment and BoxShade for annotating amino acid similarities. Analysis of leader peptides was performed with online SignalP 4.1Server (Petersen and others ), and PSORT II analysis of nuclear localization signals and intracellular localization was performed with PSORT server (http://psort.hgc.jp/) (Nakai and Horton ). Statistical analysis and graphical presentation of results were done with GraphPad Prism 6 (GraphPad). […]

Pipeline specifications

Software tools Clustal W, BoxShade, SignalP, PSORT
Applications Protein sequence analysis, Proteome data visualization
Organisms Homo sapiens
Diseases Hepatitis C