Dataset features

Specifications

Application:	Gene expression microarray analysis
Number of samples:	23
Release date:	Mar 23 2010
Last update date:	Mar 22 2012
Access:	Public
Chemicals:	Atrazine
Dataset link	Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans: atrazine

Experimental Protocol

Adults of C. elegans strain GE-31 exposed as biological replicate groups (approx 10,000) to a control and 4 concentrations of atrazine from L1 stage. Replicate populations were sampled 12 hours after the on-set of egg laying and hybridised against a common reference for purposes of normalisation. All experiments were conducted following a reference design with the reference sample compiled from a mixture of RNA extracted from control and cadmium-, fluoranthene-, atrazine- and copper-exposed worms from L1, L4 and adult life-stages. Use of this reference was intended to provide optimal coverage of the spotted genes.