Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: Mar 23 2010
Last update date: Mar 22 2012
Access: Public
Chemicals: Atrazine
Dataset link Linking toxicant physiological mode of action with induced gene expression changes in Caenorhabditis elegans: atrazine

Experimental Protocol


Adults of C. elegans strain GE-31 exposed as biological replicate groups (approx 10,000) to a control and 4 concentrations of atrazine from L1 stage. Replicate populations were sampled 12 hours after the on-set of egg laying and hybridised against a common reference for purposes of normalisation. All experiments were conducted following a reference design with the reference sample compiled from a mixture of RNA extracted from control and cadmium-, fluoranthene-, atrazine- and copper-exposed worms from L1, L4 and adult life-stages. Use of this reference was intended to provide optimal coverage of the spotted genes.

Repositories


GEO

GSE21008

ArrayExpress

E-GEOD-21008

BioProject

PRJNA129655

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Contact


Peter Kille

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