|Application:||Gene expression microarray analysis|
|Number of samples:||16|
|Release date:||Apr 26 2006|
|Last update date:||Aug 28 2018|
|Dataset link||Expression data for validation of single cell cDNA amplification method (V1V3 method)|
To verify the ability of the developed method, we prepared total RNA from ES cells and diluted it to the single-cell level (10 pg). Eight array data from the cDNA samples amplified independently from the 10 pg RNA (amplified samples) were compared to each other, and to eight array data from the undiluted 5 ug RNA (nonamplified controls). Since all the materials were the same, all of the observed variations were attributable to the methodogoly.
Citations per year
Nucleic Acids Res