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Affinity purification-mass spectrometry data analysis software tools | MS based proteomics targeted proteomics

Identifying the interactions established by a protein of interest can be a critical step in understanding its function. This is especially true when an unknown protein of interest is demonstrated to physically interact with proteins of known function. While many techniques have been developed to characterize protein–protein interactions, one strategy that has gained considerable momentum over the past decade for identification and quantification of protein–protein interactions, is affinity-purification followed by mass spectrometry (AP-MS).

Source text:
(Dunham et al., 2012) Affinity-purification coupled to mass spectrometry: Basic principles and strategies. Proteomics.

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