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Protocols

Ag1000g specifications

Information


Unique identifier OMICS_24937
Name Ag1000g
Alternative name Anopheles gambiae 1000 genomes
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Not allowed
Maintained Yes

Maintainer


  • person_outline Alistair Miles

Additional information


https://www.malariagen.net/projects/ag1000g#data-use

Ag1000g citations

 (4)
library_books

Preferential suppression of Anopheles gambiae host sequences allows detection of the mosquito eukaryotic microbiome

2017
Sci Rep
PMCID: 5468309
PMID: 28607435
DOI: 10.1038/s41598-017-03487-1

[…] is currently still referenced as A. gambiae, without distinguishing between the two new species. To confirm the applicability of the Silva database entry for both species, we used vcf files from the Anopheles gambiae 1000 Genomes Consortium project to extract the V4 and V9 region from whole-genome sequences of both A. gambiae and A. coluzzii individuals, and sequences were aligned using Jalview ( […]

library_books

Massive introgression drives species radiation at the range limit of Anopheles gambiae

2017
Sci Rep
PMCID: 5394460
PMID: 28417969
DOI: 10.1038/srep46451

[…] eration and sequencing were undertaken according to the manufacturer’s protocol for paired-end 100 bp sequence reads. In addition, a sample from Antula was retrieved from the genomes available at the Anopheles gambiae 1000 genomes consortium. Additional A. gambiae and also A. coluzzii specimens were sequenced but did not yield sufficient quality sequence for inclusion after conservative filtering […]

call_split

The ace 1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications

2016
PLoS Biol
PMCID: 5137868
PMID: 27918584
DOI: 10.1371/journal.pbio.2000618
call_split See protocol

[…] individuals) and Acerduplikis (DD, eight individuals) samples were sequenced by the Wellcome Trust Sanger Institute, as part of the Malaria genome project (https://www.malariagen.net/projects/vector/ag1000g). FASTQ format libraries were generated from 100 bp-read pairs separated by a 250 bp insert. These reads were mapped to the An. gambiae PEST reference genome assembly, downloaded from VectorBa […]

library_books

Evolutionary Dynamics of Abundant Stop Codon Readthrough

2016
Mol Biol Evol
PMCID: 5100048
PMID: 27604222
DOI: 10.1093/molbev/msw189

[…] cataloging candidates in that species.We also compared recent purifying selection in ancient A. gambiae readthrough regions to those in the comparison group, using single nucleotide variants from the Anopheles gambiae 1000 genomes project (). Within ancient readthrough regions, 45.4% of variants are synonymous (2,814 of 6,198), whereas in the comparison readthrough regions only 42.6% are (3,091 of […]

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