AGEseq specifications


Unique identifier OMICS_12675
Name AGEseq
Interface Web user interface
Restrictions to use None
Input data One sequence read file
Programming languages Perl
Computer skills Basic
Stability Stable
Maintained Yes




  • person_outline Liang-Jiao Xue

Publication for AGEseq

AGEseq citation


CRISPRing into the woods

PMCID: 5033219
PMID: 26357840
DOI: 10.1080/21645698.2015.1091553

[…] ou et al., ). By designing consensus primers flanking the target site, we were able to sequence both 4CL1 and 4CL5 amplicons simultaneously to assess the specificity of CRISPR/Cas9. A custom program, AGEseq (Analysis of Genome Editing by sequencing), was developed to facilitate variant detection (Xue and Tsai, ). No off-target cleavage in the 4CL5 locus was detected in 4CL1-edited lines, suggestin […]

AGEseq institution(s)
School of Forestry and Natural Resources, University of Georgia, Athens, GA, USA; Department of Genetics, University of Georgia, Athens, GA, USA; Institute of Bioinformatics, University of Georgia, Athens, GA, USA
AGEseq funding source(s)
This work was supported by the Georgia Research Alliance-Haynes Forest Biotechnology endowment.

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