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AGEseq specifications


Unique identifier OMICS_12675
Name AGEseq
Interface Web user interface
Restrictions to use None
Input data One sequence read file
Programming languages Perl
Computer skills Basic
Stability Stable
Maintained Yes




  • person_outline Liang-Jiao Xue <>

Publication for AGEseq

AGEseq citation


CRISPRing into the woods

PMCID: 5033219
PMID: 26357840
DOI: 10.1080/21645698.2015.1091553

[…] et al., ). by designing consensus primers flanking the target site, we were able to sequence both 4cl1 and 4cl5 amplicons simultaneously to assess the specificity of crispr/cas9. a custom program, ageseq (analysis of genome editing by sequencing), was developed to facilitate variant detection (xue and tsai, ). no off-target cleavage in the 4cl5 locus was detected in 4cl1-edited lines, […]

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AGEseq institution(s)
School of Forestry and Natural Resources, University of Georgia, Athens, GA, USA; Department of Genetics, University of Georgia, Athens, GA, USA; Institute of Bioinformatics, University of Georgia, Athens, GA, USA
AGEseq funding source(s)
This work was supported by the Georgia Research Alliance-Haynes Forest Biotechnology endowment.

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