Align RGB planes statistics

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Align RGB planes specifications


Unique identifier OMICS_25186
Name Align RGB planes
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Version 1.7
Stability Stable
Maintained Yes




No version available


  • person_outline Gabriel Landini

Additional information

Align RGB planes citations


Probing protein interactions in living mammalian cells on a microtubule bench

Sci Rep
PMCID: 4661529
PMID: 26610591
DOI: 10.1038/srep17304
call_split See protocol

[…] s stage, we analyzed whether, although using achromatic lenses, the red and green images were not shifted with respect to each other. Such shift, if any, can be corrected using the ImageJ’s Plug-In, “Align RGB planes” (). To quantify the colocalization level between a protein bait fused to Tau and putative protein preys, we adapted a method previously described. Both images were then filtered usin […]


Super Resolution Fluorescence of Huntingtin Reveals Growth of Globular Species into Short Fibers and Coexistence of Distinct Aggregates

ACS Chem Biol
PMCID: 4273975
PMID: 25330023
DOI: 10.1021/cb500335w
call_split See protocol

[…] were plotted with 2D Gaussian functions of constant amplitude and σ = 50 nm. The SR reconstructions were then overlaid using the TetraSpeck bead signals in both images and aligned in ImageJ with the Align_RGB_planes plugin (G. Landini). […]


Cannabinoid Type 1 Receptors Transiently Silence Glutamatergic Nerve Terminals of Cultured Cerebellar Granule Cells

PLoS One
PMCID: 3922925
PMID: 24533119
DOI: 10.1371/journal.pone.0088594

[…] 12 pixels for all the images. Spurious ROIs were manually removed. The mask generated in the FM1-43 experiment using Igor Pro software was superimposed onto the immunocytochemistry images using the “Align RGB planes” tool. The ROIs located at the edges of the image, did not exactly match the immunoreactive puncta and they were therefore excluded from the quantification. To appropriately quantify […]


Studying synaptic efficiency by post hoc immunolabelling

BMC Neurosci
PMCID: 3854067
PMID: 24138605
DOI: 10.1186/1471-2202-14-127

[…] action on the images acquired using a 12-pixel ball radius []. Take the FM1-43 image as a reference and after merging the channels (Image > Color > Merge Channels), align the images using the Image J Align RGB planes plug-in ( Once aligned, split the channels (Image > Color > Split channels) and save the aligned images for posterior […]


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