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AMUSER specifications

Information


Unique identifier OMICS_04796
Name AMUSER
Alternative names Automated DNA Modifications with USER cloning, PHUSER
Interface Web user interface
Restrictions to use None
Input data DNA sequence(s)
Input format FASTA
Computer skills Basic
Version 1.0
Stability Stable
Maintained Yes

Maintainers


  • person_outline Rasmus Wernersson
  • person_outline Lars Ronn
  • person_outline Hans Jasper Genee

Publications for Automated DNA Modifications with USER cloning

AMUSER citations

 (3)
library_books

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

2015
Sci Rep
PMCID: 4676018
PMID: 26657798
DOI: 10.1038/srep18016

[…] e remaining hα1AT and hC1INH constructs () were cloned by seamless PCR-based uracil-specific excision reagent (USER) fusion cloning, essentially as previously described, using primers designed by the AMUSER software. In brief, PCR amplicons () were made using the proofreading polymerase PfuX7 and assembled by treating with USER Enzyme (New England Biolabs) and transforming into E. coli One Shot® M […]

library_books

How does graphotactic knowledge influence children's learning of new spellings?

2013
Front Psychol
PMCID: 3790077
PMID: 24109466
DOI: 10.3389/fpsyg.2013.00701

[…] s habitants de la campagne se retrouvaient pour le muppile, la fête du village. C'était l'occasion de chanter, de danser et de déguster les spécialités de la région. Au muppile, la coutume était de s'amuser toute la nuit et d'attendre que le jour se lève pour partager un énorme gâteau au nifude. Le nifude est un fruit délicieux que l'on trouvait dans les forêts. Lors d'unmuppile, une drôle d'histo […]

library_books

Reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in Saccharomyces cerevisiae

2013
Microb Cell Fact
PMCID: 3654996
PMID: 23557488
DOI: 10.1186/1475-2859-12-31

[…] [] [FGDB: FGSG_02324] into the recipient vector BGHA-P1 [] with in vivo ligation in E. coli. All exon fragments were PCR amplified from F. graminearum PH1 genomic DNA using primers designed with the PHUSER web tool (see oligonucleotides in Additional file ) []. An internal HindIII restriction site in the ORF was silently mutated by mixing approx. 200 ng plasmid harboring the gene with 10 nmol of […]

Citations

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AMUSER institution(s)
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark; Bioinformatics Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark; The Finsen Laboratory, Rigshospitalet, Faculty of Health Sciences, Copenhagen, Denmark; Biotech Research and Innovation Center (BRIC), Copenhagen, Denmark; Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark; Department of Chemistry, Technical University of Denmark, Lyngby, Denmark; Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark; Intomics A/S, Lyngby, Denmark; Bioinformatics Centre, Department of Biology, University of Copenhagen, Copenhagen, Denmark
AMUSER funding source(s)
Supported by the Novo Nordisk Foundation.

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