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ANI Calculator specifications

Information


Unique identifier OMICS_27703
Name ANI Calculator
Alternative names Average Nucleotide Identity Calculator, OrthoANIu
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input data Some genome files.
Input format FASTA
Operating system Unix/Linux
Programming languages Java
Computer skills Advanced
Stability Stable
Requirements
USEARCH
Maintained Yes

Versioning


No version available

Maintainers


  • person_outline EzTaxon-e
  • person_outline Jongsik Chun
  • person_outline Jongsik Chun

Additional information


https://www.ezbiocloud.net/tools/orthoaniu

Information


Unique identifier OMICS_27703
Name ANI Calculator
Alternative names Average Nucleotide Identity Calculator, OrthoANIu
Interface Web user interface
Restrictions to use None
Input format FASTA
Programming languages Java
Computer skills Basic
Stability Stable
Maintained Yes

Maintainers


  • person_outline EzTaxon-e
  • person_outline Jongsik Chun
  • person_outline Jongsik Chun

Additional information


https://www.ezbiocloud.net/tools/orthoaniu

Publication for Average Nucleotide Identity Calculator

ANI Calculator citations

 (67)
call_split

Predictable Phenotypes of Antibiotic Resistance Mutations

2018
MBio
PMCID: 5954217
PMID: 29764951
DOI: 10.1128/mBio.00770-18
call_split See protocol

[…] Average nucleotide identities (ANI) were calculated as described by Goris et al. () using the ANI calculator provided by Kosta Konstantinidis (http://enve-omics.ce.gatech.edu/ani/). The minimum length of the alignment was set at 700 nucleotides (nt) with a minimum identity of 70%. The genomes […]

library_books

Genome centric metatranscriptomes and ecological roles of the active microbial populations during cellulosic biomass anaerobic digestion

2018
Biotechnol Biofuels
PMCID: 5911951
PMID: 29713376
DOI: 10.1186/s13068-018-1121-0

[…] and the PhyloPhlAn results were adopted for subsequent analyses. The genome-wide Average Nucleotide Identity (gANI) of the PGs against their neighboring reference genome was calculated using the JGI ANI calculator [] (Additional file ). The phylogenetic tree based on PhyloPhlAn results was drawn with GGTREE []. The metabolism of each PG was manually curated using KEGG annotations and annotations […]

library_books

Species Delimitation, Phylogenetic Relationships, and Temporal Divergence Model in the Genus Aeromonas

2018
Front Microbiol
PMCID: 5920023
PMID: 29731747
DOI: 10.3389/fmicb.2018.00770

[…] ho analyzed the relationships of 40 A. media strains with the recently proposed species of A. rivipollensis. These authors also identified two groups of strains inside the A. media cluster with ANIb (ANI calculator and EzGenome) and isDDH values between groups of ≤94.6% and ≤61.2%, respectively. These values, which are below the considered threshold for isolates of the same species, support the ex […]

library_books

Chromobacterium spp. mediate their anti Plasmodium activity through secretion of the histone deacetylase inhibitor romidepsin

2018
Sci Rep
PMCID: 5906607
PMID: 29670144
DOI: 10.1038/s41598-018-24296-0

[…] sequence deposited for C. violaceum ATCC 12472 (NCBI RefSeq NC_005085.1), which was used as reference for further assembly. Pairwise genome average nucleotide identity (gANI) was calculated using the OrthoANIu algorithm, and the resulting similarity matrix was used to generate an UPGMA tree using DendroUPGMA.The genomic sequence obtained for C. sp. Panama was then uploaded to antiSMASH v. 4. (bact […]

library_books

Complete Genome Sequence of Lactobacillus plantarum subsp. plantarum Strain LB1 2, Isolated from the Hindgut of European Honeybees, Apis mellifera L., from the Philippines

2018
Genome Announc
PMCID: 5887034
PMID: 29622612
DOI: 10.1128/genomeA.00209-18

[…] ttps://www.ncbi.nlm.nih.gov/genome/annotation_prok). Species identity was established by calculating the average nucleotide identity (ANI) and in silico digital DNA-DNA hybridization (dDDH) using the ANI Calculator () and the Genome-to-Genome Distance Calculator version 2.1 (), respectively, against previously sequenced genomes in the GenBank database. Secondary metabolites were predicted using an […]

library_books

Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax

2018
Genes
PMCID: 5867893
PMID: 29558455
DOI: 10.3390/genes9030172

[…] The average nucleotide identity (ANI) calculator used [] estimated the average nucleotide identity using reciprocal best hits (two-way ANI) between two genomic datasets, as calculated in []. […]

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ANI Calculator institution(s)
School of Biological Sciences & Institute of Molecular Biology & Genetics, Seoul National University, Seoul, Korea
ANI Calculator funding source(s)
Supported by the Strategic Initiative for Microbiomes in Agriculture and Food, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (Project No. 914008-04).

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