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ANOSVA specifications


Unique identifier OMICS_07625
Alternative name ANalysis Of Splice VAriation
Software type Application/Script
Interface Command line interface
Restrictions to use None
Biological technology Affymetrix
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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  • person_outline Melissa Cline <>

Publication for ANalysis Of Splice VAriation

ANOSVA in publications

PMCID: 5945577
PMID: 29467494
DOI: 10.1038/s41388-018-0129-z

[…] [] and most of the splicing analysis methods suffer from high false-positive detection rates, we combined three different analysis methods: microarray detection of alternative splicing (midas []), analysis of splice variation (anosva [, ]), and finding isoforms using robust multichip analysis (firma []) to increase the stringency (described in detail in materials and methods section). […]

PMCID: 5849159
PMID: 29560095
DOI: 10.18632/oncotarget.23039

[…] room temperature (1:10000). the target proteins were detected with ecl and grey value of bands were analyzed by image lab software., the results were expressed as means ± sd and analyzed by one-way anosva. statistical significance was defined at p < 0.05., this work is supported by the natural science foundation of hubei province (2015bca268 and 2016cfb648) and natural science foundation […]

PMCID: 5462735
PMID: 28592875
DOI: 10.1038/s41598-017-03354-z

[…] differential splicing analysis was conducted using limma. alternatively spliced probe-sets were identified using a double threshold of adjusted p-value < 1e-06 and absolute log2 fc > 2. the analysis of splice variation (anosva) method was also used as an additional filter with an adjusted p-value < 1e-06 at both the probe and probe-set levels. only protein-coding genes were finally […]

PMCID: 5445373
PMID: 28545391
DOI: 10.1186/s12859-017-1687-8

[…] the exon level expression intensities by their corresponding gene level intensities, and compares these normalized intensities between sample groups. another anova based method is the so-called analysis of splice variation (anosva) [], which fits a linear model to the observed data aiming to identify non-zero interaction terms between the sample groups and the exons. however, […]

PMCID: 4384382
PMID: 25886751
DOI: 10.1186/s13062-015-0045-x

[…] of these data and we anticipate that our data will generally be valuable in the inference of tf activities as well as in the creation of regulatory networks. , finally, i could not follow the analysis of splice variation. the authors say that the major isoform comprises 80-90% of the total expression for each protein-coding gene. are they really saying this is true for all genes? […]

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ANOSVA institution(s)
Affymetrix, Inc, 3380 Central Expressway, Santa Clara, CA, USA
ANOSVA funding source(s)
Affymetrix, Inc., Santa Clara, CA, USA; Pasteur Institute, Paris, France; Roche, Inc., Palo Alto, CA, USA; University of Rochester Medical Center, Rochester, NY, USA; Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, CA, USA

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