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Leucine Rich Repeat Kinase 2 Controls the Ca2+/Nuclear Factor of Activated T Cells/IL 2 Pathway during Aspergillus Non Canonical Autophagy in Dendritic Cells
[…] ng Technology, Danvers, MA, USA), Anti-PPP2R1A antibody [6F9] (Abcam, Cambridge, UK), GAPDH antibody, clone: 6C5 (Merck Millipore, Billerica, MA, USA), DAPK1 mouse polyclonal antibody, clone: RB3033 (antibodies-online.com). For DAPK1, normalization was performed by probing the membrane with mouse monoclonal β-actin antibody, clone: AC-40 (Sigma-Aldrich). Band pixel density was analyzed from the fi […]
Decelerated neurodegeneration after intravitreal injection of α synuclein antibodies in a glaucoma animal model
[…] were calculated per mm retina.The expression level of α-synuclein protein in the retina was investigated in retinal cross sections as described before. The rabbit α-synuclein primary Ab (ABIN1031834, antibodies-online.com) was diluted 1:100 in DPBS and incubated on six cross sections per eye overnight at 4 °C. Samples were incubated with a goat anti-rabbit IgG (H + L) Cy3 (1:1000, ZRV1158, Linaris […]
Angiopoietin‐Like 2 Promotes Atherogenesis in Mice
[…] , for 1 month. We targeted a concentration of angptl2 at equilibrium of ≈150 ng/mL, equivalent to twice the plasma concentration measured in 12‐month‐old atherosclerotic ATX mice (estimated by ELISA [antibodies‐online.com], data not shown). Mice were euthanized at 4 months of age after 1 month of infusion; the aortas were dissected to measure atherosclerotic lesions (in unstained aortas, using GIM […]
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