Antibody humanization software tools | Immune system data analysis
Different methods have been developed for increasing the mAbs ‘degree of humanness’ by replacing parts of the original non-human antibody with the corresponding human counterparts. This process is generally referred as ‘humanization’ and takes advantage of the particular architecture of the antibody molecule. The molecules generated by such humanization procedures may partially or completely lose affinity for their intended antigen; this can be usually restored by re-introducing specific and case-dependent native residues in the humanized molecule through an experimental trial-and-error procedure going under the name of ‘back-mutation’ phase.
A web server for antibody humanization. Tabhu includes tools for human template selection, grafting, back-mutation evaluation, antibody modelling and structural analysis, helping the user in all the critical steps of the humanization experiment protocol.