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Assembly PCR Oligo Maker specifications

Information


Unique identifier OMICS_08751
Name Assembly PCR Oligo Maker
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input data DNA sequence or RNA sequence
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Maintained Yes

Versioning


No version available

Publication for Assembly PCR Oligo Maker

Assembly PCR Oligo Maker citations

 (4)
library_books

Proteolysis of mature HIV 1 p6 Gag protein by the insulin degrading enzyme (IDE) regulates virus replication in an Env dependent manner

2017
PLoS One
PMCID: 5384750
PMID: 28388673
DOI: 10.1371/journal.pone.0174254

[…] in silico by using JCat (http://www.jcat.de/, []). An N-terminal Met as well as a 5’ BamHI and a 3’ EcoRI restriction site were added. Corresponding overlapping DNA oligonucleotides were designed by Assembly PCR Oligo Maker [], purchased from Biomers (Ulm, Germany), and a continuous DNA fragment was generated thereof by assembly-PCR according to previously established protocols [,] using the Vent […]

library_books

Structural Dynamics of a Mitochondrial tRNA Possessing Weak Thermodynamic Stability

2014
Biochemistry
PMCID: 3985750
PMID: 24520994
DOI: 10.1021/bi401449z

[…] imilarly. DNA for in vitro transcription containing a T7 RNA polymerase binding site, 5′ linker, tRNALeuUAA, 3′-linker, and reverse transcriptase binding site, in that order, was constructed using an Assembly PCR oligo maker. Recursive gene synthesis was performed employing VENT DNA polymerase. RNA was then prepared using in vitro transcription and gel purification. […]

library_books

New advances in leukaemia immunotherapy by the use of Chimeric Artificial Antigen Receptors (CARs): state of the art and perspectives for the near future

2011
PMCID: 3195094
PMID: 21939556
DOI: 10.1186/1824-7288-37-46

[…] . method is based on dividing the putative sequence into different overlapping oligonucleotides. To generate specific and functional overlapping oligonucleotides, they created a free online software "Assembly PCR oligo maker" http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1160141/. This software calculates the overlapping oligonucleotides following the putative sequence with specific values of T melt […]

library_books

Crystal Structure Analysis Reveals Functional Flexibility in the Selenocysteine Specific tRNA from Mouse

2011
PLoS One
PMCID: 3101227
PMID: 21629646
DOI: 10.1371/journal.pone.0020032

[…] A DNA fragment containing a T7 RNA polymerase promoter, a sequence encoding mouse tRNASec and restriction sites was PCR-assembled from DNA primers designed with the Assembly PCR Oligo Maker program . The PCR product was cloned into the pUC19 plasmid by standard techniques. The sequence of the insert was verified by DNA sequencing.The DNA template for in vitro tra […]

Citations

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Assembly PCR Oligo Maker institution(s)
Department of Chemistry, York University, Toronto, Ontario, Canada
Assembly PCR Oligo Maker funding source(s)
This work was supported by funding from the Natural Sciences and Engineering Research Council of Canada (NSERC).

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