Assembly PCR Oligo Maker specifications

Information


Unique identifier OMICS_08751
Name Assembly PCR Oligo Maker
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input data DNA sequence or RNA sequence
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Maintained Yes

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Publication for Assembly PCR Oligo Maker

Assembly PCR Oligo Maker in publications

 (3)
PMCID: 5384750
PMID: 28388673
DOI: 10.1371/journal.pone.0174254

[…] in silico by using jcat (http://www.jcat.de/, []). an n-terminal met as well as a 5’ bamhi and a 3’ ecori restriction site were added. corresponding overlapping dna oligonucleotides were designed by assembly pcr oligo maker [], purchased from biomers (ulm, germany), and a continuous dna fragment was generated thereof by assembly-pcr according to previously established protocols [,] using […]

PMCID: 3985750
PMID: 24520994
DOI: 10.1021/bi401449z

[…] dna for in vitro transcription containing a t7 rna polymerase binding site, 5′ linker, trnaleuuaa, 3′-linker, and reverse transcriptase binding site, in that order, was constructed using an assembly pcr oligo maker. recursive gene synthesis was performed employing vent dna polymerase. rna was then prepared using in vitro transcription and gel purification., to study the structural […]

PMCID: 3101227
PMID: 21629646
DOI: 10.1371/journal.pone.0020032

[…] for stable trnasec folding., a dna fragment containing a t7 rna polymerase promoter, a sequence encoding mouse trnasec and restriction sites was pcr-assembled from dna primers designed with the assembly pcr oligo maker program . the pcr product was cloned into the puc19 plasmid by standard techniques. the sequence of the insert was verified by dna sequencing., the dna template for in vitro […]


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Assembly PCR Oligo Maker institution(s)
Department of Chemistry, York University, Toronto, Ontario, Canada
Assembly PCR Oligo Maker funding source(s)
This work was supported by funding from the Natural Sciences and Engineering Research Council of Canada (NSERC).

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