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Assembly polishing software tools | De novo sequencing data analysis

Assembly polishing software tools | De novo sequencing data analysis The Pacific Biosciences RSII instrument is able to directly detect methylation from untreated DNA at both known and previously unrecognized motifs by analyzing polymerase kinetics in long sequence reads. For 5-mC, alterations in polymerase kinetics are subtle and spread over several bases, effectively precluding direct single-molecule detection.
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