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Attune NxT Software specifications

Information


Unique identifier OMICS_33493
Name Attune NxT Software
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Version 2.7
Stability Stable
Registration required Yes
Maintained Yes

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No version available

Attune NxT Software citations

 (10)
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Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP mediated recombination system

2018
PLoS One
PMCID: 5874048
PMID: 29590189
DOI: 10.1371/journal.pone.0194887
call_split See protocol

[…] c). A gate was applied to the FSC/SSC plot to exclude dead cells and debris. Doublet discrimination was performed based on the FSC-A/FSC-H plot. 20,000 events were collected. Data were analysed using Attune NxT software. Mean fluorescent intensity of EGFP-positive cells was calculated. […]

library_books

The Candida albicans stress response gene Stomatin Like Protein 3 is implicated in ROS induced apoptotic like death of yeast phase cells

2018
PLoS One
PMCID: 5794166
PMID: 29389961
DOI: 10.1371/journal.pone.0192250

[…] ve experiment.Fluorescent cells were quantified using an Attune NxT Flow Cytometer (Life Technologies) equipped with a 50 mW, 488 nm LED laser and 530/30 nm emissions filters. Data was analyzed using Attune NxT Software v2.2. Acquisition settings were set to wt untagged control cells by adjusting the voltage to the third logarithmic decade of the fluorescence channel on a histogram plot. We gated […]

library_books

The Small RNA ncS35 Regulates Growth in Burkholderia cenocepacia J2315

2018
PMCID: 5760752
PMID: 29359187
DOI: 10.1128/mSphere.00579-17

[…] Cell size and granularity of planktonic cultures and biofilms were measured with an Attune Nxt flow cytometer and autosampler from Invitrogen with the Attune Nxt software, version 5.2 (Thermo Fisher). Planktonic cultures were investigated by using a suspension with an OD of 0.1 (1 × 107 CFU/ml). For biofilms, cells were grown and detached as describ […]

library_books

Palmitic acid induces neurotoxicity and gliatoxicity in SH SY5Y human neuroblastoma and T98G human glioblastoma cells

2018
PeerJ
PMCID: 5924683
PMID: 29713567
DOI: 10.7717/peerj.4696

[…] s measured using the 530/30 nm (BL1) and 695/40 nm (BL3) emission filters, respectively, with excitation at 488 nm. A total of 10,000 events per sample was recorded. Data collected were analysed with Attune™ Nxt Software. Compensation was set up using unstained cells, cells stained with Alexa Fluor® 488 annexin V only and cells stained with PI only. Negative gating strategy involving forward scatt […]

library_books

ERV1 Overexpression in Myeloid Cells Protects against High Fat Diet Induced Obesity and Glucose Intolerance

2017
Sci Rep
PMCID: 5634420
PMID: 28993702
DOI: 10.1038/s41598-017-13185-7

[…] -Violet by excluding remaining RBCs. Doublets were excluded on forward scatter (FSC) height (H) vs. FSC width (W) dot plots (<20%). Each sample was analyzed in duplicate. Data were analyzed using the Attune NxT Software v2.4 and FlowJo v10. […]

library_books

Revealing the sequence of interactions of PuroA peptide with Candida albicans cells by live cell imaging

2017
Sci Rep
PMCID: 5333355
PMID: 28252014
DOI: 10.1038/srep43542

[…] ration of 50 μg\mL and the samples were incubated for 1 h at 4 C in the dark. Cell cycle analysis was performed using Attune NxT Flow Cytometer (Invitrogen™; USA), and the results were analyzed using Attune NxT software. […]

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Attune NxT Software institution(s)
Thermo Fisher Scientific, Waltham, MA, USA

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