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AutoDimer specifications


Unique identifier OMICS_03931
Name AutoDimer
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability No
Maintained No

Publication for AutoDimer

AutoDimer citations


Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real Time PCR

PMCID: 5788046
PMID: 29372257
DOI: 10.1167/iovs.17-22597
call_split See protocol

[…] inct DNA melting profiles, allowing differentiation of these two herpes simplex virus subtypes by Tm. Primers were further optimized to minimize potential for primer-dimer and hairpin formation using AutoDimer. […]


Using long ssDNA polynucleotides to amplify STRs loci in degraded DNA samples

PLoS One
PMCID: 5669423
PMID: 29099837
DOI: 10.1371/journal.pone.0187190

[…] In-silico analyses of the primer interactions were performed with the AutoDimer software developed by Vallone et al [] at the National Institute of Standards and Technology (NIST). All software parameters were set at default values except for the Total Strand Concentrat […]


Molecular Basis of Overdominance at a Flower Color Locus

PMCID: 5714491
PMID: 29051190
DOI: 10.1534/g3.117.300336

[…] s this view. An early study on alcohol dehydrogenase (Adh) activity in maize () revealed a molecular basis for single-gene overdominance that is similar to our results. The Adh enzyme is a dimer. The autodimer in one inbred line has high activity but low stability, whereas the autodimer in another inbred line has low activity but high stability. The allodimer formed in the heterozygote is both act […]


Development and inter laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines

Sci Rep
PMCID: 5561262
PMID: 28819142
DOI: 10.1038/s41598-017-09377-w

[…] The specificity of the assays was determined in silico. The interactions between primers and probes were determined using the Autodimer software and the multiplex specificity was predicted using the ePCR software. No significant risk of dimers between pairs of oligonucleotides (primers/probes) was observed. In multiplex PCR, […]


Developmental validation of a 6 dye typing system with 27 loci and application in Han population of China

Sci Rep
PMCID: 5498568
PMID: 28680088
DOI: 10.1038/s41598-017-04548-1

[…] gned for amplicons smaller than 500 bp using the Primer3 program ( primer3-0.4.0/primer3/input.htm). Each primer was checked for potential structures of the self dimmer using the AutoDimer v1.1 software and non-specific hybridizations in other genome regions using NCBI Basic Local Alignment Search Tool (BLAST). 27 loci were organized by expected amplicon size and assigned to f […]


Testing of microsatellite multiplexes for individual identification of Cape Parrots (Poicephalus robustus): paternity testing and monitoring trade

PMCID: 5363265
PMID: 28344897
DOI: 10.7717/peerj.2900
call_split See protocol

[…] ; multiplex 6: Prob29, Prob34, and Prob35). In-silico testing of all multiplexes was done prior to PCR amplification to test for the presence of primer dimers and hairpin structures using the program AutoDimer v1 (). The selected multiplex combinations did not show any signs of primer dimers or hairpin structures among the primer pairs. The forward primer in each microsatellite pair was fluorescen […]


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AutoDimer institution(s)
Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD, USA

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