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AutoLink specifications


Unique identifier OMICS_03380
Name AutoLink
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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Publication for AutoLink

AutoLink in publications

PMCID: 5645095
PMID: 29040282
DOI: 10.1371/journal.pone.0186271

[…] with antibodies against cytokeratin (ck) 18, β-catenin, smad4, pstat3, and the dually phosphorylated extracellular signal-regulated kinase 1 and 2 (perk1/2) using an autostainer (dako autolink 48; dako, glostrup, denmark) with dab as chromogen (details in ). nuclear β-catenin translocation was scored as follows: score 2 if nuclear (and cytoplasmic) instead of/in addition […]

PMCID: 5595878
PMID: 28900126
DOI: 10.1038/s41598-017-10508-6

[…] spots per slide). slides were blocked for 1 h with i-block reagent (applied biosystems) followed by a 15 min incubation with re-blot reagent (dako) and were loaded on an automated slide stainer autolink 48 (dako, carpinteria, ca, usa) for incubation with primary antibodies. antibody binding was detected by fluorescence with a vectastain-abc streptavidin–biotin complex (vector, pk-6100) […]

PMCID: 5444734
PMID: 28415799
DOI: 10.18632/oncotarget.16282

[…] laboratories, burlingame, ca, usa). the sections were counterstained with haemalaun and covered by a glass slip. the formalin-fixed human tissue was deparaffinized in a pt-link and stained on an autolink from dako (denmark). antibodies were used as described [, , , ]., rna from pancreata and other organs followed the procedure described earlier []. total rna was quantified by nanodrop 2000 […]

PMCID: 5165031
PMID: 28058063
DOI: 10.1007/s12254-016-0292-2

[…] platforms and different staining protocols as well as different scoring systems and different cut offs for predictive evaluation (table ; []). they can be used on ventana ultra systems, dako autolink 48 stainers or others with or without enhancement systems (figs.  and ).table 1 asome clones are only available as free antibodies and bothers as free antibodies and as kits cdepending […]

PMCID: 5064015
PMID: 27694828
DOI: 10.1038/ncomms12991

[…] of 960 lysates/slide (2880 spots/slide). the slides were processed as described and probed with a set of 200 antibodies against total and phosphoprotein proteins using an automated slide stainer autolink 48 (dako). each slide was incubated with one specific primary antibody and negative control slide was incubated with antibody diluent instead of primary antibody. primary antibody binding […]

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AutoLink institution(s)
Department of Molecular Biology and Biophysics, ETH, Zurich, Switzerland

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