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AutoQuant specifications

Information


Unique identifier OMICS_25438
Name AutoQuant
Alternative name AutoQuant X3
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Input format XML,AQH,TIF,TIFF,RAW,DEB,AVZ,BMP,SEQ,ICS,IDS,PIC,IMS,LEI,LIF,OIF,OIB,ZVI,LSM,CZI,ND2,IPL,IPLAB,ND,STK
Operating system Windows
Computer skills Medium
Stability Stable
Registration required Yes
Maintained Yes

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Documentation


AutoQuant citations

 (271)
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Quantifying compressive forces between living cell layers and within tissues using elastic round microgels

2018
Nat Commun
PMCID: 5951850
PMID: 29760452
DOI: 10.1038/s41467-018-04245-1
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[…] Before traction calculation, 3D confocal images were deconvoluted (AutoQuant X3), and the separated free nanoparticles from the microgel were discarded using pencil tool in ImageJ (NIH). This step will enhance the image quality and prevent artifacts in the recovered […]

library_books

Chromosome Segregation Is Biased by Kinetochore Size

2018
Curr Biol
PMCID: 5954971
PMID: 29706521
DOI: 10.1016/j.cub.2018.03.023

[…] acquisition (0.22 μm thick z stacks) was performed on a Zeiss AxioObserver Z1 wide-field microscope equipped with a plan-apochromatic (1.46 NA 60x) DIC objective and a cooled CCD (Hamamatsu Orca R2). Autoquant X (Media Cybernetics) was used for blind deconvolution. All images show maximum intensity projections. For classification of kinetochore-microtubule attachments, microtubules were traced thr […]

library_books

Src Kinase Dependent Rapid Non genomic Modulation of Hippocampal Spinogenesis Induced by Androgen and Estrogen

2018
Front Neurosci
PMCID: 5938344
PMID: 29765299
DOI: 10.3389/fnins.2018.00282

[…] images with super resolution confocal microscopic imaging (Figure ) than those with conventional confocal microscopic imaging (Figure ) which uses blind deconvolution method with AutoDeblur software (AutoQuant, USA). Particularly necks of spines were clearly observed in Airy Scan mode, while sometimes spine heads spatially isolated from dendrites, without visible necks which connect spine heads to […]

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Polyphosphate Accumulating Bacteria: Potential Contributors to Mineral Dissolution in the Oral Cavity

2018
Appl Environ Microbiol
PMCID: 5861820
PMID: 29352083
DOI: 10.1128/AEM.02440-17
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[…] objective with a numerical aperture of 1.42. polyP and DNA signals were separated based on a standard polyP emission wavelength (550 nm) and DNA emission wavelength (461 nm).Images were analyzed with AutoQuant X (v. X3.0.4) and Imaris x64 (v. 7.5.2). Blind three-dimensional deconvolution with default settings (adaptive point spread function [PSF], 10 iterations, and medium noise) was employed in A […]

library_books

Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals

2018
PMCID: 5944365
PMID: 29551411
DOI: 10.1016/j.cub.2018.02.032

[…] All 4D live-imaging data were deconvolved with either ImageJ (National Institute of Health) plug-in Microvolution (Microvolution, 2014–2016) or AutoQuant 3X (Media Cybernetics) using adaptive PSF (blind) prior to analysis. Data were processed using Imaris (Bitplane), ImageJ (National Institute of Health), Amira 6.3 (FEI - Thermo Fisher Scient […]

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PDE4D regulates Spine Plasticity and Memory in the Retrosplenial Cortex

2018
Sci Rep
PMCID: 5832851
PMID: 29497131
DOI: 10.1038/s41598-018-22193-0
call_split See protocol

[…] h resolution (0.10 × 0.10 × 0.33 μm voxels). Target neurons were identified by anatomical location and cell morphology. Microscopy was performed blind to experimental conditions. Blind deconvolution (AutoQuant) was applied to raw three‐dimensional digital images which were then analyzed for spine density and morphology. Individual spines were measured manually for head diameter, length, and neck t […]

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AutoQuant institution(s)
Media Cybernetics

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