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AutoQuant specifications


Unique identifier OMICS_25438
Name AutoQuant
Alternative name AutoQuant X3
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
Computer skills Medium
Stability Stable
Registration required Yes
Maintained Yes


No version available


AutoQuant citations


Quantifying compressive forces between living cell layers and within tissues using elastic round microgels

Nat Commun
PMCID: 5951850
PMID: 29760452
DOI: 10.1038/s41467-018-04245-1
call_split See protocol

[…] Before traction calculation, 3D confocal images were deconvoluted (AutoQuant X3), and the separated free nanoparticles from the microgel were discarded using pencil tool in ImageJ (NIH). This step will enhance the image quality and prevent artifacts in the recovered […]


Chromosome Segregation Is Biased by Kinetochore Size

Curr Biol
PMCID: 5954971
PMID: 29706521
DOI: 10.1016/j.cub.2018.03.023

[…] acquisition (0.22 μm thick z stacks) was performed on a Zeiss AxioObserver Z1 wide-field microscope equipped with a plan-apochromatic (1.46 NA 60x) DIC objective and a cooled CCD (Hamamatsu Orca R2). Autoquant X (Media Cybernetics) was used for blind deconvolution. All images show maximum intensity projections. For classification of kinetochore-microtubule attachments, microtubules were traced thr […]


Src Kinase Dependent Rapid Non genomic Modulation of Hippocampal Spinogenesis Induced by Androgen and Estrogen

Front Neurosci
PMCID: 5938344
PMID: 29765299
DOI: 10.3389/fnins.2018.00282

[…] images with super resolution confocal microscopic imaging (Figure ) than those with conventional confocal microscopic imaging (Figure ) which uses blind deconvolution method with AutoDeblur software (AutoQuant, USA). Particularly necks of spines were clearly observed in Airy Scan mode, while sometimes spine heads spatially isolated from dendrites, without visible necks which connect spine heads to […]


Polyphosphate Accumulating Bacteria: Potential Contributors to Mineral Dissolution in the Oral Cavity

Appl Environ Microbiol
PMCID: 5861820
PMID: 29352083
DOI: 10.1128/AEM.02440-17
call_split See protocol

[…] objective with a numerical aperture of 1.42. polyP and DNA signals were separated based on a standard polyP emission wavelength (550 nm) and DNA emission wavelength (461 nm).Images were analyzed with AutoQuant X (v. X3.0.4) and Imaris x64 (v. 7.5.2). Blind three-dimensional deconvolution with default settings (adaptive point spread function [PSF], 10 iterations, and medium noise) was employed in A […]


Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals

PMCID: 5944365
PMID: 29551411
DOI: 10.1016/j.cub.2018.02.032

[…] All 4D live-imaging data were deconvolved with either ImageJ (National Institute of Health) plug-in Microvolution (Microvolution, 2014–2016) or AutoQuant 3X (Media Cybernetics) using adaptive PSF (blind) prior to analysis. Data were processed using Imaris (Bitplane), ImageJ (National Institute of Health), Amira 6.3 (FEI - Thermo Fisher Scient […]


PDE4D regulates Spine Plasticity and Memory in the Retrosplenial Cortex

Sci Rep
PMCID: 5832851
PMID: 29497131
DOI: 10.1038/s41598-018-22193-0
call_split See protocol

[…] h resolution (0.10 × 0.10 × 0.33 μm voxels). Target neurons were identified by anatomical location and cell morphology. Microscopy was performed blind to experimental conditions. Blind deconvolution (AutoQuant) was applied to raw three‐dimensional digital images which were then analyzed for spine density and morphology. Individual spines were measured manually for head diameter, length, and neck t […]


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AutoQuant institution(s)
Media Cybernetics

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