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AutoQuant specifications

Information


Unique identifier OMICS_25438
Name AutoQuant
Alternative name AutoQuant X3
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Input format XML,AQH,TIF,TIFF,RAW,DEB,AVZ,BMP,SEQ,ICS,IDS,PIC,IMS,LEI,LIF,OIF,OIB,ZVI,LSM,CZI,ND2,IPL,IPLAB,ND,STK
Operating system Windows
Computer skills Medium
Stability Stable
Registration required Yes
Maintained Yes

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AutoQuant in pipelines

 (12)
2017
PMCID: 5461764
PMID: 28587682
DOI: 10.1186/s40478-017-0444-6

[…] laser scanning microscope (leica dmi 6000b microscope and a leica tcs sp2 aobs confocal laser scanning imaging system (leica microsystems ag). afterwards, acquired images were deconvoluted using autoquant x3 software (media cybernetics inc., rockville, md, usa) and loaded into imaris imaging software (bitplane, zurich, switzerland). image segmentation was used to discriminate protrusions […]

2017
PMCID: 5886076
PMID: 28973161
DOI: 10.1093/hmg/ddx316

[…] .5 μm) and images were acquired using volocity software. each image shown represents a stack of 10 images spaced 0.2 μm apart in the z-plane, for each wavelength. image stacks were deconvoluted using autoquant (media cybernetics, bethesda, md, u.s.a.) software (using the manufacturer supplied psf adapted for spinning disk microscopy). analysis of the stacks was performed using imaris (bitplane, zu […]

2016
PMCID: 4946219
PMID: 27441280
DOI: 10.1016/j.heliyon.2016.e00109

[…] on a leica mps60 for paraffin sections and on a leica dm rxa for oct sections. acquisitions were performed using metamorph (molecular devices) software. deconvoluted 3d pictures were obtained from autoquant x3 (mediacybernetics) and imaris (bitplan) softwares., the colorectal cancer cell lines ht29, hct116 and sw480 were originally obtained from the atcc and used from frozen stocks at low […]

2016
PMCID: 5005324
PMID: 27630827
DOI: 10.3389/fcimb.2016.00090

[…] images were collected at 0.5−1 μm incremental depths using scanimage (janelia farms, hhmi). images were saved in a single tiff file with 16 bit depth. deconvolution of the images was performed using autoquant × 3 and 3d models of z-stacks were generated using imaris software., animal experiments were conducted in compliance with the regulations and standards under the animal welfare act, […]

2016
PMCID: 5042578
PMID: 27489338
DOI: 10.1091/mbc.E16-03-0159

[…] camera and filters were controlled by metamorph (molecular devices, sunnyvale, ca). image stacks were collected at 0.2-μm steps through the whole cell volume and then deconvolved using autodeblur (autoquant imaging, troy, ny) for 10 or 20 iterations., to quantify kinetochore number in individual cells, the image stacks obtained from microscopy were imported into imaris three-dimensional (3d) […]


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AutoQuant in publications

 (390)
PMCID: 5951850
PMID: 29760452
DOI: 10.1038/s41467-018-04245-1

[…] alone. all samples were imaged under 37 °c and 5% co2 controlled by a mounted incubator chamber (okolab stage top incubator)., before traction calculation, 3d confocal images were deconvoluted (autoquant x3), and the separated free nanoparticles from the microgel were discarded using pencil tool in imagej (nih). this step will enhance the image quality and prevent artifacts in the recovered […]

PMCID: 5954971
PMID: 29706521
DOI: 10.1016/j.cub.2018.03.023

[…] (0.22 μm thick z stacks) was performed on a zeiss axioobserver z1 wide-field microscope equipped with a plan-apochromatic (1.46 na 60x) dic objective and a cooled ccd (hamamatsu orca r2). autoquant x (media cybernetics) was used for blind deconvolution. all images show maximum intensity projections. for classification of kinetochore-microtubule attachments, microtubules were traced […]

PMCID: 5938344
PMID: 29765299
DOI: 10.3389/fnins.2018.00282

[…] with super resolution confocal microscopic imaging (figure ) than those with conventional confocal microscopic imaging (figure ) which uses blind deconvolution method with autodeblur software (autoquant, usa). particularly necks of spines were clearly observed in airy scan mode, while sometimes spine heads spatially isolated from dendrites, without visible necks which connect spine heads […]

PMCID: 5916877
PMID: 29707402
DOI: 10.1038/s41413-018-0014-y

[…] using silica impregnated filter paper (pall corporation, port washington, ny). the itlc was run in 50 mmol · l−1 edta, ph 5, and subsequently imaged and quantified using the phosphorimager and the autoquant software package, respectively (packard, perkinelmer, hopkinton, ma). for both specific sac55 and control antibodies, specific activities were in the range of 1.7–2.2 mci · g−1., […]

PMCID: 5944365
PMID: 29551411
DOI: 10.1016/j.cub.2018.02.032

[…] nm for cy3, and 645 nm for cy5 signal acquisitions., all 4d live-imaging data were deconvolved with either imagej (national institute of health) plug-in microvolution (microvolution, 2014–2016) or autoquant 3x (media cybernetics) using adaptive psf (blind) prior to analysis. data were processed using imaris (bitplane), imagej (national institute of health), amira 6.3 (fei - thermo fisher […]


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