Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 1
Release date: Feb 23 2010
Last update date: Dec 6 2012
Access: Public
Diseases: Deficiency Diseases
Dataset link Non adherent macrophage of mafb null and WT

Experimental Protocol


Macrophages were derived from day 14.5 fetal livers of mafB- /- and WT mice. Suspensions of single fetal liver cells were prepared by mechanical disruption . A total of 106 cells in suspension were centrifuged at 1,200 rpm for 5 min, and the cell pellet was resuspended in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (heat inactivated), streptomycin and penicillin (100 units/ml), and macrophage colony-stimulating factor (M-CSF) (10 ng/ml) and then seeded either onto a nonadhesive dishes coated with hydrophilic polymers (Hydrocell; Cell Seed, Tokyo). The culture medium was not changed throughout the experiment. M-CSF (final concentration, 10 ng/ml) was added every day from day 4 onwards. One, 2, 4, and 6 days after seeding, the cells were harvested and analyzed by flow cytometry. After 6 day culture, macrophages of Mafb-/- and WT were use microarray analysis .

Repositories


GEO

GSE20419

ArrayExpress

E-GEOD-20419

BioProject

PRJNA125497

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Contact


Michito Hamada