Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Nov 20 2009
Last update date: Aug 15 2018
Access: Public
Chemicals: Amino Acids, Nitrogen, Salicylic Acid
Dataset link Differential gene expression profiling from Arabidopsis thaliana wild type (Columbia-0) and lht1 mutant leaves

Experimental Protocol


We used microarrays to detail the global profile of gene expression after knocking out the LHT1 gene and identified distinct classes of up- and down-regulated genes associated with the lht1 mutation. The Arabidopsis plants grown for 3 weeks in short day condition, without or with 48hr pathogen infection, were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of leaves in order to better represent expression profiles. To that end, we hand-selected the leaves at the same stages (fully expanded) and mixed them together for each biological replicate.

Repositories


GEO

GSE19109

ArrayExpress

E-GEOD-19109

BioProject

PRJNA120461

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