Computational protocol: Vascularization of the dorsal root ganglia and peripheral nerve of the mouse: Implications for chemical-induced peripheral sensory neuropathies

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Protocol publication

[…] Laser scanning confocal microscopy of the whole mount preparations was performed with a BX-61 microscope equipped with the Fluoview 1000 imaging software 5.0 (Olympus America Inc, Melville, NY). Confocal z-series at 0.5 μm intervals were acquired for each observation area and filtered by two-frame Kalman low-speed scans. YFP was excited by a laser wavelength of 488 nm, and emissions were detected using a 522-nm emission filter. Sequential acquisition mode was used to reduce bleed through. Z-series of the different experiments were imported from the Olympus Fluoview format to the Imaris Pro Software 5.7.0 (Bitplane AG, Zurich, CH). Image threshold and channel pseudocolors were adjusted, and 3D reconstruction was performed in the Surpass module. Images were cropped with Adobe Photoshop CS and thereafter assembled in Adobe Illustrator CS. […]

Pipeline specifications

Software tools Imaris, Adobe Illustrator
Applications Miscellaneous, Laser scanning microscopy
Organisms Mus musculus, Homo sapiens
Diseases Neoplasms, Nervous System Diseases, Peripheral Nervous System Diseases, HIV Infections, Neurotoxicity Syndromes