Computational protocol: Characterisation of Major Histocompatibility Complex Class I in the Australian Cane Toad, Rhinella marina

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Protocol publication

[…] Sequences were edited in BioEdit 7 and confirmed to contain MHC sequences using tblastx (available online at http://blast.ncbi.nlm.nih.gov/Blast.cgi; ). Codons were aligned by ClustalW as implemented in MEGA5 . MEGA was also used to construct maximum likelihood phylogenies from the nucleotide sequences, using General Time Reversible model of nucleotide substitution as recommended by Modelgenerator and rooted on their midpoints. […]

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