Computational protocol: Relationship between neuropsychological impairment and grey and white matter changes in adult-onset myotonic dystrophy type 1

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Protocol publication

[…] The automated analysis of T1 structural data was carried out by FSL-VBM (), an optimised VBM protocol () carried out with FMRIB software library package (FSL) (). As preprocessing step T1 images were corrected for WM lesions using the lesion filling toolbox available in FSL (). Structural images were brain-extracted using BET (Brain Extraction Tool) (), and then they were automatically segmented into GM, WM and cerebrospinal fluid (CSF) tissue-type by FAST4 tool (). The GM volume images were aligned to the Montreal Neurological Institute (MNI) 152 standard space () by the affine registration tool FLIRT (), followed by non-linear registration using FNIRT (). The registered GM images of an equal number of healthy controls and DM1 patients were averaged and flipped along the x-axis to create a left-right symmetric, study-specific grey matter template. After that all native GM images were non-linearly registered to this study-specific template, modulated and smoothed with an isotropic Gaussian kernel with a sigma of 3 mm.A voxel-wise General Linear Model (GLM) was applied using permutation-based non-parametric testing (5000 permutations), correcting for multiple comparisons across space (p < 0.001) by threshold-free cluster enhancement (TFCE) option (). Since voxel based quantification of atrophy is influenced by aging () we inserted age and gender of patients and controls as covariate variables within the GLM matrix.In order to establish a possible relationship with VBM changes multiple regression analyses were also performed inserting neuropsychological scores in different GLM design matrix, using age and gender as covariates of no interest (TFCE p < 0.05 corrected for multiple comparisons).The description of statistical maps obtained from VBM analysis was based on Anatomical Automated Labeling (AAL) Atlas (). […]

Pipeline specifications

Software tools FSL, BET, AAL
Application Magnetic resonance imaging
Organisms Homo sapiens
Diseases Lymphoma, Non-Hodgkin, Myotonic Dystrophy