|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Jul 3 2013|
|Last update date:||Aug 23 2018|
|Diseases:||Granulosa Cell Tumor|
|Dataset link||Laser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries|
Laser microdissection was used to collect a purified population of granulosa cells only from preovulatory follicles. We chose to compre the response to PMSG or PMSG+hCG of granulosa cells collected from either WT and bERKO preovulatory follicles. We chose to collect cells 48h after mice were treated with PMSG to compare the gene expression profile ot preovulatory granulosa cells. We also studied the response of these cells to LH (or hCG) as we collected cells 4h after mice were treated with hCG (peak of transcriptional response to hCG).
NIEHS Microarray Core