Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jul 3 2013
Last update date: Aug 23 2018
Access: Public
Diseases: Granulosa Cell Tumor
Chemicals: Estrogens
Dataset link Laser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries

Experimental Protocol


Laser microdissection was used to collect a purified population of granulosa cells only from preovulatory follicles. We chose to compre the response to PMSG or PMSG+hCG of granulosa cells collected from either WT and bERKO preovulatory follicles. We chose to collect cells 48h after mice were treated with PMSG to compare the gene expression profile ot preovulatory granulosa cells. We also studied the response of these cells to LH (or hCG) as we collected cells 4h after mice were treated with hCG (peak of transcriptional response to hCG).

Repositories


GEO

GSE44651

ArrayExpress

E-GEOD-44651

BioProject

PRJNA190701

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Contact


NIEHS Microarray Core