Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Apr 1 2018
Last update date: Oct 10 2018
Access: Public
Diseases: Lung Diseases, Lung Neoplasms, Neoplasms, Tumor Lysis Syndrome
Dataset link Transcriptional and Epigenomic profile of GSK126 or dox-mediated Ezh2 inhibition in KrasG12D/+;Trp53-/-;Ezh2i-GFP-2A-rTA;Luc lung tumors in vivo

Experimental Protocol


We generated mRNA profiles of tumor cells tail vein injected into the lungs of Nude mice by deep sequencing. After FACS purification, RNA extraction and Bioanalyzer analysis, we processed only samples with high quality cellular and RNA profiles. Overall, we compared 10-day GSK126 treated cells (n=4) and up to 30 days GSK126 treated cells (n=3) to Captisol-treated samples (vehicle, n=2), using Illumina Hiseq2000. FACS sorted cells from individual animals were obtained by GFP expression. For H3K27ac and H2AK5ac profiling, we used KP primary tumors generated by injection of NSCLC into the tail vein of nude mice. Mice were sacrificed on the onset of shortness of breath and tissues were resuspended in ChIP lysis buffer.

Repositories


GEO

GSE61125

ENA

SRP046252

BioProject

PRJNA260292

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Contact


Gaetano Gargiulo