Computational protocol: The Development of Diet-Induced Obesity and Glucose Intolerance in C57Bl/6 Mice on a High-Fat Diet Consists of Distinct Phases

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Protocol publication

[…] The 2-DE gels were analysed using Progenisis Samespots software (Nonlinear Dynamics Ltd, UK). Spots which showed differences in normalised average volume with P<0.05 by Anova in HF vs. LF were cut from SDS-PAGE gels. Gel plugs were trypsinized using the MassPrep Station (Waters, Micromass, UK) protocol. Spot identification was carried out by LC/MS/MS as described previously using an ‘Ultimate’ nanoLC system (LC Packings, UK) and a Q-Trap (Applied Biosystems/MDS Sciex, UK) triple quadrupole mass spectrometer fitted with a nanospray ion source. The total ion current (TIC) data were submitted for database searching using the MASCOT search engine (Matrix Science, UK) using the MSDB database. […]

Pipeline specifications

Software tools SameSpots, Mascot Server
Application MS-based untargeted proteomics
Organisms Mus musculus
Diseases Liver Diseases, Muscular Diseases, Glucose Intolerance, Neoplasms, Adipose Tissue
Chemicals Glucose