Dataset features


Application: Gene expression microarray analysis
Number of samples: 22
Release date: Dec 31 2014
Last update date: Dec 31 2014
Access: Public
Diseases: Osteogenesis Imperfecta
Chemicals: Formaldehyde
Dataset link Gene expression profiles of vaccinated fish using a long-term efficiency mixed vaccine against Edwardsiella tarda performed by a new designed oligo DNA microarray in Japanese flounder Paralichthys olivaceus

Experimental Protocol

The kidney were isolated from the vaccinated Japanese flounder using the three formalin-killed vaccines, 1st is a typical type of E. tarda strain UH6 isolated from Japanese flounder, Paralichthys olivaceus, 2nd is a atypical type of E. tarda strain UT1 isolated from Red seabream, Pagrus major and 3rd is an equal (1:1) mixture FKC with these two type E. tarda. Fishes were vaccinated by intramuscular injection using the 1.0 x 10^8 cell of FKC vaccines. After 3 and 7 days from a vaccination, fish kidney was isolated. We also isolated non-vaccinated fish kidney as a control. We analyzed a four of individual fish kidney vaccinated using mixed FKC on day 7. We also analyze the three samples which are the two of the pooled total RNA from a two fish and the one of the one fish total RNA in typical FKC and atypical FKC vaccinated fish after 3 and 7 days from a vaccination, mixed FKC vaccinated fish after day 3 from a vaccination and a control (22 hybridization).








Hidehiro Kondo