Computational protocol: Plasma amino acids imbalance in cirrhotic patients disturbs the tricarboxylic acid cycle of dendritic cell

Similar protocols

Protocol publication

[…] Raw data obtained by CE-TOFMS were processed with MasterHands. Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database (Human Metabolome Technologies Inc., Japan) based on their migration times (MTs) and m/z values determined by TOFMS. The tolerance range for the peak annotation was configured at ±0.5 min for MTs and ±10 ppm for m/z. In addition, peak areas were normalized against those of the internal standards and then the resultant relative area values were further normalized by sample amount. [...] The data were analyzed with ANOVA, and multiple comparisons were performed with Dunnett's post-hoc procedure. When 2 groups were analyzed, the differences between groups were analyzed by the Wilcoxon t-test. All data are expressed as mean SEM. In all analyses, a P value of less than 0.05 was considered statistically significant. All statistical analyses were performed with standard statistical software (JMP® Pro 9 for Windows). […]

Pipeline specifications

Software tools MasterHands, JMP Pro
Applications Miscellaneous, MS-based untargeted metabolomics
Organisms Homo sapiens
Chemicals Adenosine Triphosphate, Glucose