Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 27 2012
Last update date: Mar 28 2013
Access: Public
Dataset link Brucella melitensis-infected HeLa cells gene expression

Experimental Protocol


We generated 4 different samples: A) RNA isolated from Brucella melitensis-infected HeLa cells at 4 h p.i.; B) RNA isolated from non-infected HeLa cells at 4 h p.i. of the A samples; C) RNA isolated from Brucella melitensis-infected HeLa cells at 12 h p.i., and D) RNA isolated from non-infected HeLa cells at 12 h p.i. of the D samples. The B. melitensis-infected HeLa cells gene expression was compared with gene expression from non-infected HeLa cells treated similarly. There are four biological replicates of each sample and time point (n=16). ORFs were single spotted on each microarray. Each replicate was normalized against labeled universal human reference RNA.

Repositories


GEO

GSE14703

ArrayExpress

E-GEOD-14703

BioProject

PRJNA112203

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Contact


Leslie Adams
Leslie Garry Adams