Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 104
Release date: May 24 2017
Last update date: Nov 28 2017
Access: Public
Diseases: Diabetic Cardiomyopathies
Chemicals: Calcium
Dataset link Molecular phenotyping empowers drug discovery: a proof-of-concept study

Experimental Protocol


Molecular Phenotyping after 12h of compound treatment; A total number of 80 compounds were profiled with molecular phenotyping, along with four control conditions: media control, which represents the healthy status; GEC, which mimicks the stress condition in T2D patients; BM, a cocktail of bosentan, an enthothelin receptor antagonist, and mifepristone, a progesterone receptor antagonist, which is supposed to reverse the disease phenotype; and GEC+BM, which mimicks a successful reversal of the phenotype from the stress condtion. The compounds were screened on six plates (A-F), with one replicate for each compound and one replicate for each control on each plate. Some compounds that failed the first screening run were tested in two cherry-picking replication plates (R1 and R2). Calcium channel inhibitor hits were then tested in phenotypic high-content microscopy assays with two or three biological replicates each.

Repositories


GEO

GSE89972

ENA

SRP093740

BioProject

PRJNA354350

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Contact


Jitao David Zhang