|Number of samples:||104|
|Release date:||May 24 2017|
|Last update date:||Nov 28 2017|
|Dataset link||Molecular phenotyping empowers drug discovery: a proof-of-concept study|
Molecular Phenotyping after 12h of compound treatment; A total number of 80 compounds were profiled with molecular phenotyping, along with four control conditions: media control, which represents the healthy status; GEC, which mimicks the stress condition in T2D patients; BM, a cocktail of bosentan, an enthothelin receptor antagonist, and mifepristone, a progesterone receptor antagonist, which is supposed to reverse the disease phenotype; and GEC+BM, which mimicks a successful reversal of the phenotype from the stress condtion. The compounds were screened on six plates (A-F), with one replicate for each compound and one replicate for each control on each plate. Some compounds that failed the first screening run were tested in two cherry-picking replication plates (R1 and R2). Calcium channel inhibitor hits were then tested in phenotypic high-content microscopy assays with two or three biological replicates each.
Jitao David Zhang