Dataset features


Application: DNA methylation array analysis
Number of samples: 24
Release date: Feb 19 2017
Last update date: Nov 27 2018
Access: Public
Chemicals: Nicotine
Dataset link In utero exposure to maternal smoking is associated with DNA methylation alterations and reduced neuronal content in the developing fetal brain

Experimental Protocol

The Illumina Infinium HM450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 480,000 CpGs in 24 fetal samples dissected from the cortical plate in the region that becomes the DLPFC in order to obtain post-migratory NeuN-immunoreactive (NeuN+) neurons in the second trimester of gestation. Samples include 10 fetal cortical sections from mothers with no reported smoking prior to or during pregnancy and 14 fetal cortical sections from mothers that reported smoking prior to and during pregnancy. Genomic DNA was extracted using the QIAamp DNA Mini Kit (Qiagen) following standard instructions and bisulfite converted (Zymo). Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol








Zac Chatterton

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