|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Aug 25 2017|
|Last update date:||Aug 25 2017|
|Dataset link||The Increased Toxicity of UV-Degraded Nitroguanidine and IMX-101 Exposures in Zebrafish Larvae: Evidence Implicating Oxidative Stress |
Six separate microarray analyses were conducted within this study. The 6 designs were conduced as follows: 1. NQ parent compound, 2. UV-Treated NQ, 3. Two-way design, NQ Parent x UV-Treated NQ, 4. IMX-101 parent material, 5. UV-treated IMX-101, 6. Two way design, IMX-101 parent x UV-Treated IMX-101. This GEO entry represents analysis number 1. - NQ parent compound: Zebrafish larvae were exposed to NQ at 0 (control), 1.4, 2.8, 5.5, 11.0, 22.7, 45.6, 90.4, 181, 348, and 732 mg/L (measured concentrations). Exposure and control water was dechlorinated tap water (Vicksburg, MS USA municipal dechlorinated via activated carbon filtration) amended with artificial sea salts (Instant Ocean, Blacksburg, VA, USA) to a conductivity of 600 µS/cm. Danio rerio larvae were exposed in static nonrenewal acute 96-h bioassays. Exposure chambers were 300-ml high-form lipless beakers with a test solution volume of 250 ml. Eight larval fish per beaker were exposed in the parent or UV-treated NQ treatments each including 4 exposure replicates and 6 control replicates. Larval zebrafish were fed newly hatched Artemia nauplii two hours before the initiation of exposures and at the 48-h timepoint. Surviving fish were enumerated daily and any fish found to be deceased were promptly removed from exposure chambers.