Dataset features

Specifications


Application: miRNA array analysis
Number of samples: 3
Release date: Feb 27 2018
Last update date: Feb 28 2018
Access: Public
Taxon: Homo sapiens
Dataset link Whole genome pooled shRNA screen to characterize Raptinal-induced apoptosis in the MIA PaCa-2 cell line

Experimental Protocol


Cells were treated with Raptinal (1.25-1.8 micromolar) or DMSO vehicle control over a 20 day period. After every 2-3 days of drug treatment, cells were allowed to recover in drug-free medium and treatment repeated for a total of 3 rounds over the 20 day period. Two biological replicates of Raptinal-treatment were performed and one biological replicate of DMSO vehicle control was performed. Cell passaging was performed on a scale sufficient to maintain >100 fold library representation. At the end of the 20 days, total RNA was extracted and shRNA abundances determined.

Repositories


GEO

GSE66423

BioProject

PRJNA276889

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Contact


Kathryn Partlow