|Application:||miRNA array analysis|
|Number of samples:||3|
|Release date:||Feb 27 2018|
|Last update date:||Feb 28 2018|
|Dataset link||Whole genome pooled shRNA screen to characterize Raptinal-induced apoptosis in the MIA PaCa-2 cell line|
Cells were treated with Raptinal (1.25-1.8 micromolar) or DMSO vehicle control over a 20 day period. After every 2-3 days of drug treatment, cells were allowed to recover in drug-free medium and treatment repeated for a total of 3 rounds over the 20 day period. Two biological replicates of Raptinal-treatment were performed and one biological replicate of DMSO vehicle control was performed. Cell passaging was performed on a scale sufficient to maintain >100 fold library representation. At the end of the 20 days, total RNA was extracted and shRNA abundances determined.